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phosphate buffered saline dpbs solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phosphate buffered saline dpbs solution
    Phosphate Buffered Saline Dpbs Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphate+buffered+saline+dpbs+solution/Phosphate-buffered+saline+(DPBS%2C+1X)%2C+Dulbecco's+formula/pm42174303-51-22-26
    Average 99 stars, based on 1 article reviews
    phosphate buffered saline dpbs solution - by Bioz Stars, 2026-09
    99/100 stars

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    Saline:

    Article Title: Biocompatible injectable glycyrrhizic acid-based growth factor hydrogel for synergistic endometrial regeneration
    Article Snippet: .. As for PDGF-BB release, the hydrogel was dispersed into test tubes containing 3 mL Dulbecco’s phosphate-buffered saline (DPBS) solution (Gibco BRL, Eggenstein, Germany) [26]. ..

    Article Title: Three-Dimensional Bioprinted Gelatin—Genipin Hydrogels Enriched with hUCMSC-Derived Small Extracellular Vesicles for Regenerative Wound Dressings
    Article Snippet: The contact angle analysis performed using the ImageJ application (V1.5, Bethesda, MD, USA) revealed the hydrophilic or hydrophobic nature of the samples, which influences cell attachment and proliferation. .. The biodegradability of the hydrogels was analyzed by immersing the samples in 0.0006 mg/mL collagenase type I (Worthington-Biochemical Corporation, Lakewood, NJ, USA) in Dulbecco’s phosphate-buffered saline (DPBS) solution (Gibco, San Diego, CA, USA) at physiological temperature (37 °C) for 24 h. The weight loss of the hydrogels over time served as an indicator of enzymatic breakdown, simulating the in vivo microenvironment. ..

    Article Title: Host-pathogen interactions during early stages of bovine mastitis: divergent macrophage responses to distinct bovine-associated Staphylococci species and strains
    Article Snippet: .. After bacterial growth, the bacterial suspension was centrifuged at 2500 × g for 15 min at 4 °C, and washed twice with sterile Dulbecco’s phosphate-buffered saline (DPBS) solution without calcium chloride and magnesium chloride (cat. no. 14190185, Gibco, Paisley, United Kingdom). .. Afterward, the bacteria were resuspended in antibiotic-free RPMI-1640 cell culture medium (cat. no. R7638, Sigma Aldrich, St. Louis, USA).

    Article Title: Habitat Type and Locality Structure the Midgut Microbiota of Aedes albopictus.
    Article Snippet: .. Briefly, each mosquito was rinsed three times in sterile water, surface disinfected in 70% ethanol for 5 min, washed in sterile Dulbecco’s phosphate-buffered saline (DPBS) solution (ThermoFisher, Waltham, MA, USA) for 5 min, and then subjected to five additional rinse cycles in sterile DPBS, with each cycle lasting 3 min. Each surface-cleaned Ae. albopictus was placed in a drop of sterile DPBS under a stereo dissecting microscope and the midgut was harvested. .. Midguts were individually suspended in 1.5-mL microcentrifuge tubes containing 50 μL of RNAlater solution (ThermoFisher, Waltham, MA, USA) and stored at -80 C until DNA isolation.

    Article Title: Fabrication of PCL Blended Highly Aligned Nanofiber Yarn from Dual-Nozzle Electrospinning System and Evaluation of the Influence on Introducing Collagen and Tropoelastin
    Article Snippet: .. Next, these samples were immersed in 20 mL of Dulbecco’s phosphate-buffered saline (dPBS) solution (Gibco Life Technologies, Gaithersburg, MD, USA) in individual 50 mL centrifuge tubes. .. The tubes were capped and placed in a Boekel Grant shaker bath operating at a continuous speed of 20 rpm and a temperature of 37 ± 1 °C, for a designated time period.

    Article Title: Host-pathogen interactions during early stages of bovine mastitis: divergent macrophage responses to distinct bovine-associated Staphylococci species and strains.
    Article Snippet: .. After bacterial growth, the bacterial suspension was centrifuged at 2500 × g for 15 min at 4 °C, and washed twice with sterile Dulbecco’s phosphate-buffered saline (DPBS) solution without calcium chloride and magnesium chloride (cat. no. 14190185, Gibco, Paisley, United Kingdom). .. Afterward, the bacteria were resuspended in antibiotic-free RPMI-1640 cell culture medium (cat. no. R7638, Sigma Aldrich, St. Louis, USA).

    Article Title: The Development of a Multilayer Transdermal Patch Platform Based on Electrospun Nanofibers for the Delivery of Caffeine
    Article Snippet: Polyethylene oxide (PEO, Mw 200,000 Da) was purchased from the Dow Chemical Company (Montgomeryville, PA, USA), and poly-ε-caprolactone (PCL, Resomer C212) was supplied by Evonik Industries (Essen, Germany), while Eucalyptus Globulus Leaf Oil (EUC, 80–85% of 1,8-cineole) was supplied by Gran Velada (Aragon, Spain). .. Dulbecco’s phosphate-buffered saline (DPBS) solution without calcium and magnesium ions was purchased from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Article Title: Three-Dimensional Bioprinted Gelatin-Genipin Hydrogels Enriched with hUCMSC-Derived Small Extracellular Vesicles for Regenerative Wound Dressings.
    Article Snippet: .. Enzymatic Biodegradation The biodegradability of the hydrogels was analyzed by immersing the samples in 0.0006 mg/mL collagenase type I (Worthington-Biochemical Corporation, Lakewood, NJ, USA) in Dulbecco’s phosphate-buffered saline (DPBS) solution (Gibco, San Diego, CA, USA) at physiological temperature (37 ◦C) for 24 h. The weight loss of the hydrogels over time served as an indicator of enzymatic breakdown, simulating the in vivo microenvironment. ..

    In Vivo:

    Article Title: Three-Dimensional Bioprinted Gelatin—Genipin Hydrogels Enriched with hUCMSC-Derived Small Extracellular Vesicles for Regenerative Wound Dressings
    Article Snippet: The contact angle analysis performed using the ImageJ application (V1.5, Bethesda, MD, USA) revealed the hydrophilic or hydrophobic nature of the samples, which influences cell attachment and proliferation. .. The biodegradability of the hydrogels was analyzed by immersing the samples in 0.0006 mg/mL collagenase type I (Worthington-Biochemical Corporation, Lakewood, NJ, USA) in Dulbecco’s phosphate-buffered saline (DPBS) solution (Gibco, San Diego, CA, USA) at physiological temperature (37 °C) for 24 h. The weight loss of the hydrogels over time served as an indicator of enzymatic breakdown, simulating the in vivo microenvironment. ..

    Article Title: Three-Dimensional Bioprinted Gelatin-Genipin Hydrogels Enriched with hUCMSC-Derived Small Extracellular Vesicles for Regenerative Wound Dressings.
    Article Snippet: .. Enzymatic Biodegradation The biodegradability of the hydrogels was analyzed by immersing the samples in 0.0006 mg/mL collagenase type I (Worthington-Biochemical Corporation, Lakewood, NJ, USA) in Dulbecco’s phosphate-buffered saline (DPBS) solution (Gibco, San Diego, CA, USA) at physiological temperature (37 ◦C) for 24 h. The weight loss of the hydrogels over time served as an indicator of enzymatic breakdown, simulating the in vivo microenvironment. ..

    Suspension:

    Article Title: Host-pathogen interactions during early stages of bovine mastitis: divergent macrophage responses to distinct bovine-associated Staphylococci species and strains
    Article Snippet: .. After bacterial growth, the bacterial suspension was centrifuged at 2500 × g for 15 min at 4 °C, and washed twice with sterile Dulbecco’s phosphate-buffered saline (DPBS) solution without calcium chloride and magnesium chloride (cat. no. 14190185, Gibco, Paisley, United Kingdom). .. Afterward, the bacteria were resuspended in antibiotic-free RPMI-1640 cell culture medium (cat. no. R7638, Sigma Aldrich, St. Louis, USA).

    Article Title: Host-pathogen interactions during early stages of bovine mastitis: divergent macrophage responses to distinct bovine-associated Staphylococci species and strains.
    Article Snippet: .. After bacterial growth, the bacterial suspension was centrifuged at 2500 × g for 15 min at 4 °C, and washed twice with sterile Dulbecco’s phosphate-buffered saline (DPBS) solution without calcium chloride and magnesium chloride (cat. no. 14190185, Gibco, Paisley, United Kingdom). .. Afterward, the bacteria were resuspended in antibiotic-free RPMI-1640 cell culture medium (cat. no. R7638, Sigma Aldrich, St. Louis, USA).

    Sterility:

    Article Title: Host-pathogen interactions during early stages of bovine mastitis: divergent macrophage responses to distinct bovine-associated Staphylococci species and strains
    Article Snippet: .. After bacterial growth, the bacterial suspension was centrifuged at 2500 × g for 15 min at 4 °C, and washed twice with sterile Dulbecco’s phosphate-buffered saline (DPBS) solution without calcium chloride and magnesium chloride (cat. no. 14190185, Gibco, Paisley, United Kingdom). .. Afterward, the bacteria were resuspended in antibiotic-free RPMI-1640 cell culture medium (cat. no. R7638, Sigma Aldrich, St. Louis, USA).

    Article Title: Habitat Type and Locality Structure the Midgut Microbiota of Aedes albopictus.
    Article Snippet: .. Briefly, each mosquito was rinsed three times in sterile water, surface disinfected in 70% ethanol for 5 min, washed in sterile Dulbecco’s phosphate-buffered saline (DPBS) solution (ThermoFisher, Waltham, MA, USA) for 5 min, and then subjected to five additional rinse cycles in sterile DPBS, with each cycle lasting 3 min. Each surface-cleaned Ae. albopictus was placed in a drop of sterile DPBS under a stereo dissecting microscope and the midgut was harvested. .. Midguts were individually suspended in 1.5-mL microcentrifuge tubes containing 50 μL of RNAlater solution (ThermoFisher, Waltham, MA, USA) and stored at -80 C until DNA isolation.

    Article Title: Host-pathogen interactions during early stages of bovine mastitis: divergent macrophage responses to distinct bovine-associated Staphylococci species and strains.
    Article Snippet: .. After bacterial growth, the bacterial suspension was centrifuged at 2500 × g for 15 min at 4 °C, and washed twice with sterile Dulbecco’s phosphate-buffered saline (DPBS) solution without calcium chloride and magnesium chloride (cat. no. 14190185, Gibco, Paisley, United Kingdom). .. Afterward, the bacteria were resuspended in antibiotic-free RPMI-1640 cell culture medium (cat. no. R7638, Sigma Aldrich, St. Louis, USA).

    Microscopy:

    Article Title: Habitat Type and Locality Structure the Midgut Microbiota of Aedes albopictus.
    Article Snippet: .. Briefly, each mosquito was rinsed three times in sterile water, surface disinfected in 70% ethanol for 5 min, washed in sterile Dulbecco’s phosphate-buffered saline (DPBS) solution (ThermoFisher, Waltham, MA, USA) for 5 min, and then subjected to five additional rinse cycles in sterile DPBS, with each cycle lasting 3 min. Each surface-cleaned Ae. albopictus was placed in a drop of sterile DPBS under a stereo dissecting microscope and the midgut was harvested. .. Midguts were individually suspended in 1.5-mL microcentrifuge tubes containing 50 μL of RNAlater solution (ThermoFisher, Waltham, MA, USA) and stored at -80 C until DNA isolation.



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